International Journal of Clinical Pharmacology & Toxicology (IJCPT)    Special Issue on "Chemical Toxicity and Pharmacology"    IJCPT-2167-910X-S3-001

Toxicity and Anxiolytic Property of Nettle in Mice in Light/Dark Test

Doukkali Z1*, Taghzouti K2, Bouidida El H3, Kamal R1, El Jemeli M1, Bahia B1, Zellou A1, Cherrah Y1, Alaoui K1

1 Pharmacodynamy Research Team, ERP, Laboratory of Pharmacology and Toxicology, Faculty of Medicine and Pharmacy, University Mohammed V of Rabat .Morocco.
2 Laboratory of Animal Physiology, Department of Biology - Faculty of Science - University Mohammed V of Rabat, Morocco.
3 National Laboratory of Drug Control, Directorate of Pharmacy, Ministry of Health, Rabat, Morocco.

*Corresponding Author

Zouhra Doukkali,
Pharmacodynamy Research Team, ERP,
Laboratory of Pharmacology and Toxicology,
Faculty of Medicine and Pharmacy, University Mohammed V of Rabat,

Received: January 22, 2016; Accepted: March 16, 2016; Published: March 29, 2016

Citation: Doukkali Z et al., (2016) Toxicity and Anxiolytic Property of Nettle in Mice in Light/Dark Test. Int J Clin Pharmacol Toxicol. S3:001, 1-6. doi:

Copyright: Doukkali Z© 2016. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution and reproduction in any medium, provided the original author and source are credited.


Background: Anxiety is an unpleasant state of inner turmoil often accompanied by cognitive, somatic, emotional, and behavioral components. There is some evidence in traditional medicine for the effectiveness of Urtica urens in the treatment of anxiety in humans. The present study was designed to study anxiolytic property of aqueous extracts of Urtica urens; an important and commonly used for its medicinal properties belongs to urticaceae family.

Methods: The anxiolytic activity was evaluated with the adult mice by hole board test, and the light-dark box test, and motor coordination with the rota rod test. The efficacy of the plant extract (100–400 mg/kg) was compared with the standard anxiolytic drug diazepam (1 mg/kg i.p.).

Results: The extract increased the time spent in the brightly-lit chamber of the light/dark box, as well as in the number of times the animal crossed from one compartment to the other. Performance on the rota rod was unaffected. In the hole board test, the extract significantly increased both head-dip counts and head-dip duration. Urtica urens, in contrast to diazepam, had no effect on locomotion.

Conclusions: These results provides support for anxiolytic activity of Urtica urens, in line with its medicinal traditional use, and may also suggest a better side-effect profile of Urtica urens relative to diazepam.

3.Material and Methods
    3.2.Plant material
    3.3.Preparation of the methanolic extract
    3.5.Treatment schedule
    3.6.Acute toxicity study
    3.7.Light/dark test
    3.6.Hole board test
    3.6.Rota rod test
    3.7.Statistical analysis
    4.1.Acute toxicity study
    4.2.Light/dark test
    4.3.Hole board test
    4.4.Rota rod test
7.Authors’ contributions


Anxiety; Urtica urens; Rota Rod Test; Hole Board Test; Light-Dark Test; Morocco.


Anxiety disorders became a very important area of research interest in psychopharmacology; Anxiety is defined as “a state of intense apprehension, uncertainty, and fear resulting from the anticipation of a threatening event or situation, often to a degree that normal physical and psychological functioning is disrupted” [1]. Emotional, cognitive, behavioral, and physical components can all be present in anxiety.

The currently treatments produce various systemic side effects and exhibit dependence and tolerance on chronic treatment which now have become a major concern about the use of currently used medicines. Benzodiazepines are the major class of compounds used in anxiety and they have remained the most commonly prescribed treatment for anxiety [2]. Although these compounds remain the mainstay of drug treatment in anxiety disorders, their side-effects are prominent, such as sedation, myorelaxation, ataxia, amnesia, and pharmacological dependence [2].

Other anti-anxiety medications include antidepressants, buspirone and b-blockers which though effective in many cases, also possess side effects like nausea, light headedness, dizziness, headache, dry mouth, constipation, diarrhea, etc. [3]. Those limitations of therapeutically show that the search for new therapeutic strategies is necessary.

Herbs are widely accepted sources of medicine, which play an important role in health care, programme worldwide [4]. Natural anxiolytic agents feature in such research because herbs have been used to treat psychiatric disorders and generally have fewer harmful effects [5].

Moreover, Moroccan climate and favored geographical location have contributed to the diversity of medicinal plants. In Morocco,four species of Urtica which belongs to the Urticaceae family are available [6]. Urtica urens commonly known as a herbaceous annual plant species of the genus Urtica, and has long been known to have tranquilizing effects among the Moroccan people [7]. This Small nettle (Urtica urens) has anti-inflammatory effect [8], nettle and their hybrids or mixtures are recommended for symptomatic treatment of rheumatoid arthritis or osteoarthritis and for increased dieresis [9]. Main constituents identified in the plant material are flavonoids, caffeoyl-esters, caffeic acid, scopoletin (cumarin), sitosterol (−3-O-glucoside), polysaccharides, fatty acids (e.g. 13- hydroxyoctadecatrienoic acid), minerals (herba: up to 20%; leaves: 1–5%) [10, 11].

Therefore, the aim of the present study was designed to characterize the anxiolytic-like activity of aqueous extract prepared from aerial part of Urtica urens. For this, elevated plus maze and open field tests, classic experimental models used to investigate the anxiolytic activity and emotional behaviors of rodents were employed.

Material and Methods


Adult mice (20–30 g) of either sex were used for the study. The animals were acquired from the animal experimental centre of Mohammed V souissi University, Medicine and pharmacy Faculty, Rabat. The animals were maintained in a room with controlled temperature (25 ± 1°C) and lighting (light/dark 12:12 h in polypropylene cages, with food and water ad libitum. Animals were acclimatized to laboratory conditions at least 1 h prior to initiation of experiments. The animals were divided into four groups, each consisting of six mice, implemented in all sets of experiments.

Plant material

The aerial part of Urtica urens was collected from the north of Morocco near the town of Wazzan (Jaaouna el Basra), with assistance of a traditional medical practitioner. The plant was authenticated by botanists of scientific institute Pr. M. Ibn Tatou and Pr. Halim Khammar. A voucher specimen (N° RAB78983) was deposited in the Herbarium of Botany Department of the Scientific Institute of Rabat.

Preparation of the methanolic extract

The aerial part was dried at room temperature and crushed. 700 g of plant material was extracted with six liter of methanol by maceration at room temperature (25°C) over period of 48 hours. Methanol containing the extract was then filtered through Whatman paper and the solvent was vacuum-distilled at 60°C in a rotary evaporator. The remaining extract was finally dried by desiccator. Final extract was a dark green paste, with 11.92% dry weight. The residue was dissolved in water for final suitable concentrations.


The methanolic extract of Urtica urens was suspended in distilled water. Diazepam (ampoule 10 mg/2 ml), pharmacy of Avicennawas diluted with saline to the required concentration before use. It is well known that benzodiazepines act as anxiolytics at low doses and that they induce sedation and muscle relaxant effects at higher doses [12]. Therefore, we used diazepam (1 mg/kg) as a positive control for anxiolytic-like effects.

Treatment schedule

Experimental groups of mice were treated orally (p.o.) with methanolic extract of Urtica urens at doses of (100–400 mg/kg), Diazepam (1 mg/kg), whereas control groups received normal saline by the same routes. All drugs were freshly prepared before each experiment. The doses of extracts were calculated to administer 0.25 ml of the suspension of extracts to the mice of 20 g. The trial was carried out 30 min after the treatments. The anxiolytic activity was examined by using the light/dark box test and the hole board test, and motor coordination test assessed with the rota rod test.

Acute toxicity study

The procedure was followed as per OECD 423 guidelines [13,20] (OECD/OCDE. 2002). The extract was administered orally at a dose of 2000 mg/kg body weight. Mice were kept under observed for 14 days to register possible mortality; their weights were registered and study their behavioral neurological toxicity.

Light/dark test

Although the light/dark test was based on the initial model described by Crawley and Goodwin (1980) [14], many authors have used it with several structural modifications. Another modified model is the one used by Belzung et al. (1987) [15].

The apparatus consisted of two 20 cm × 10 cm × 14 cm plastic boxes: one light compartment painted white and brightly illuminated and the other was dark painted black and dimly illuminated with red light. The mice were allowed to move from one box to the other through an open door between the two boxes. The illumination in the black compartment was 50 lux, in the white area it was increased to 1000 lux, generated by an extra light source. A mouse was put into the light box facing the hole. The transition between the light and the dark box and time spent in the light box were recorded for 5 min.

Hole board test

The Hole board test [16] was adopted in this test. It is made of gray Perspex. The LETICA board (signo 720; Printer LE 3333) of dimensions 40 cm × 40 cm, contained 16 evenly spaced holes (3 cm diameter and 2.2 cm depth), with in-built infra-red sensors was used for the study. The matt finishing of the upper panel avoids reflections which may alter the animal behavior. An animal was placed in the center of the hole board and allowed to freely explore the apparatus for 5 min. The number of times an animal dipped its head into the holes was automatically counted and recorded by the instrument [17].

Rota rod test

The effect on motor coordination was assessed using a rota-rod apparatus (LE 8500). Rota rod consisted of a base plant form and an iron rod of 3 cm diameter and 30 cm length, with a nonslippery surface. The rod was divided into four equal sections by three disks. The animals were pre-selected in a training session 24 h before the test, based on their ability to remain on the bar (at 12 rpm) for 2 min, and then allowing four mice to walk on the rod at the speed of 12 rpm at the same time observed over a period of 30, 60, and 90 min. Intervals between the mounting of the animal on the rotating bar and falling off of it were registered automatically as the performance time. Time spent in the apparatus was observed for 5 min duration (300 s). Apparatus was cleaned thoroughly between trials with water. All behavioral recordings were carried out with the observer blind to the treatment the mice had received.

Statistical analysis

All results are expressed as mean ± standard error of the mean. The data were analyzed statistically using one way analysis of variance ANOVA, followed by the Tukey post hoc test for multiple comparisons. P < 0.05 was taken to be statistically significant. Results were presented as tables.


Acute toxicity study

Following oral administration aqueous extract of Urtica urens at a dose of 2000 mg/kg, P.O., animals were observed for signs of toxicity such as convulsions, hypothermia, hyperactivity, and grooming continuously for 2 h and for mortality up to 24 h after administration of the doses. No toxicity and no significant changes in the body weight were observed between the treated and control group (Figure 1).

Figure 1. WC: Mice receive saline, WT : Mice receive 2000 mg/kg.

Light/dark test

Urtica urens at the dose of 200 mg/kg and diazepam (1 mg/kg) induced a significant increment of the time spent by mice on the illuminated side of the apparatus compared to the respective control group (P < 0.05, P < 0.01), without significantly affecting other parameters (Figure 2, 3).

Figure 2. Effects of aqueous extract of Urtica urens 100, 200, 400 and 600 mg/kg on time spent on light box (s).

Figure 3. Effects of aqueous extract of Urtica urens 100, 200, 400 and 600 mg/kg on number of transition in the dark test.

Hole board test

The dose 400 mg/kg of the plant extract significantly increased the number of head dippings as compared to control animals (Figure 4).

Figure 4. Effects of aqueous extract of Urtica urens 100, 200, 400 and 600 mg/kg on number of head dippings in Hole Board Test.

Rota rod test

The data shows that on average the mice treated with 100, 200 and 400 mg/kg p.o. of the methanolic extract of Urtica urens were able to maintain equilibrium on the rotating rod and stayed on longer without falling (Figure 5). Whereas diazepam (at 1 mg/kg only) showed a significant decrease in the locomotor score when compared to other groups.

Figure 5. Effects of aqueous extract of Urtica urens 100, 200, 400 and 600 mg/kg on Time (sec) of anim als remained without falling from rod.


The study analysed the therapeutic potential of aqueous extract of aerial part of Urtica urens in experimental models of anxiety in mice. The U.urens extract showed promising anxiolytic effects without causing any neuromuscular side effects.

In the therapy of anxiety disorder or acute anxiety symptoms, a combination of therapeutic interventions is mostly indicated. Beside a psychotherapeutic approach, anxiolytics are a part of treatment of anxiety [18]. Dysregulation of the GABAergique, serotoninergic, dopaminergic and adrenergic neurosystems have all been implicated in the pathophysiology of anxiety [19]. Benzodiazepines are the most widely prescribed for the last 40 years to treat several forms of anxiety; however, they have prominent side effects such as sedation, myorelaxation, ataxia and amnesia, and can cause pharmacological dependence [2]. Other anti-anxiety medications include antidepressants, buspirone and β-blockers which though effective in many cases, also possess side effects like nausea, light headedness, dizziness, headache, dry mouth, constipation, diarrhea, etc. [3].

There is considerable interest in the development of new anxiolytics, new therapies for the treatment of anxiety disorders are necessary, and the study of medicinal plants could provide new therapeutic options [20].

This work examined the anxiolytic effects of aqueous extract of Urtica urens, using the light/ dark test and the hole board, and to examine motor coordination we used Rota rod test. Furthermore, the effects of Urtica urens and diazepam on these animal models were compared to determine whether the behavioral profile Urtica urens differed from an established anxiolytic drug.

In the light/dark test, anxiety is generated by the conflict between the tendency to explore and the initial tendency to avoid the unfamiliar [14] and can be evaluated according to the number of transitions in to and the time spent in the light chamber [21,22] where in increase in these parameters is considered to reflect anxiolytic-like properties. Our results showed that aqueous extract of U. urens (400 mg/kg) increased time spent in the light chamber, suggesting anxiolytic property.

The hole board test is useful for modeling anxiety in animals, in this test an anxiolytic-like state may be reflected by an increase in head-dipping behaviors [23, 24]. Our results showed that aqueous extract (400 mg/kg) of Urtica urens increased the head dipping corroborating the anxiolytic-like effect previously shown in the light-dark test.

Rota rod test a classical animal model used to evaluate peripheral neuromuscular blockade and the motor coordination [25], a deficit in motor coordination would very likely affect performance in the behavioral tests. Our findings showed that Urtica urens (100-400 mg/kg), unlike diazepam (1 mg/kg), had no significant effect on motor coordination. Furthermore, the extract didn’t affect motor coordination in dose which produce anxiolytic effect, is additional evidence of centrally mediated actions and not blockade of neuromuscular system [16, 26]. The Urtica urens extract showed promising anxiolytic effects without causing any neuromuscular side effects.


The data presented hereby reinforce the traditional use of U.urens [7] and indicate that the aqueous extract of the aerial parts of Urtica urens L. has anxiolytic-like effects. Thus, Urtica urens may be a promising candidate for future development as a new anxiolytic drug. Further pharmacological investigations are underway to identify the active constituents of the plant extract responsible for the showed activity. Knowing the precise mechanism of how these extracts function and at what molecular targets they act should give us insights into how to develop better mood aids based on the natural products.

Authors’ contributions

DZ, I carried out all the studies and drafted the manuscript with the help of the above authors, as regards TK participated in this work and drafted with me the manuscript. EHB helped us in the chemistry part and RK, MJ, BB, carried out the behavioral tests with me, AZ help me in toxicity, and CY is the director of the laboratory he advises me and guides me always in my work, after my PhD supervisor KA she corrects the manuscript, guides me and advises me. All authors read and approved the final manuscript.


Authors are grateful to Dr. Hamid Khammar, botanist of scientific institute, Rabat, Hanae Hosni, Amina Bounihi, for their encouragement and interest in the research work.


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