Clinical, Biochemical and Bacteriological Investigation of Pneumonia in Calves with Special Reference to Alpha-1-Acid Glycoprotein Response
Almujalli AM1, El-Deeb WM1,2*,Eljalii EM1, Fouda TA1, AlBlwy M3
1 Department of clinical studies, College of Veterinary Medicine and animal Resources, King Faisal University, Saudi Arabia.
2 Department of Veterinary Medicine, infectious diseases and fish diseases, Faculty of Veterinary Medicine, Mansoura University, Mansoura, Egypt.
3 Ministry of agriculture, Department of Animal resources, Riyadh, Saudi Arabia.
Wael M. El-Deeb,
Department of clinical studies,
College of Veterinary Medicine and animal Resources,
King Faisal University, Al-Ahsa, 31982 P.O. Box: 1757,
Tel: (00966) (050) 9296154
Fax: (00966) (03) 5816635
E-mail: firstname.lastname@example.org and email@example.com
Article Type: Research article
Received: April 07, 2015; Accepted: May 15, 2015; Published: May 20, 2015
Citation: Almujalli AM, El-Deeb WM, Eljalii EM, Fouda TA, AlBlwy M (2015) Clinical, Biochemical and Bacteriological Investigation of Pneumonia in Calves with Special Reference to Alpha-1-Acid Glycoprotein Response. Int J Vet Health Sci Res. 3(5), 60-63. doi: dx.doi.org/10.19070/2332-2748-1500015
Copyright: El-Deeb WM © 2015. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution and reproduction in any medium, provided the original author and source are credited.
In order to investigate clinical and biochemical parameters in calves with bovine respiratory disease (BRD), twenty-five Holstein calves with clinical picture of BRD were selected to this investigation. Ten clinically healthy calves were selected as a control group. Blood, nasal and bronchoalveolar lavage were obtained from all calves under investigation. Complete blood parameters picture were investigated. Serum total protein, albumin, Triglyceride (TAG), High Density Lipoprotein cholesterol (HDL-c), low-density lipoprotein cholesterol (LDL-c), Total cholesterol, Alanine Amino Transferase (ALT), Aspartate Amino Transferase (AST), and Alkaline Phosphatase (ALP) were examined. Alpha-1-acid glycoprotein (AGP) was estimated in all calves under investigation. Bacteriological examination showed Pasteurella spp in 18 calves and Escherichia coli in seven calves. The laboratory results revealed a significant (P ≤ 0.05) increase in the levels of white blood cells and neutrophilia in calves with pneumonia when compared with control groups. Moreover, there was a significant (P ≤ 0.05) increase in the values of TAG, VLDL-c, LDL-c, ALT, AST, ALP and AGP with significant (P ≤ 0.05) decrease in the levels of total protein, albumin, cholesterol, HDL-c in calves with BRD when compared with control ones. From the present study, it could be concluded that AGP and lipoprotein profile could be used as diagnostic markers for BRD in calves.
3.Materials and Methods
Calves; Pneumonia; Alpha-1-acid Glycoprotein; Lipid Profile.
Bovine respiratory disease (BRD) considers one of the most imperative health problem and costly disorder happening in cattle in different localities. Amplified mortality and morbidity rates, diminished feed conversion rate, reduced feed intake, reduction in the meat quality and augmented prophylaxis and therapy lead to immense economic losses .
The most clinically detected signs of BRD include fever (about 40-41.5°C), misery, loss of appetite, nasal and ocular discharge, coughing and dyspnea of varying degrees. The causes of BRD is practically caused by different microorganisms (Mannheimia haemolytica, Pasteurella multocida, Histophilus somni, Mycoplasma bovis) and most commonly is linked with influencing risk factors related to host or environmental stressors .
The acute phase proteins (APPs) consist of proteins that display a reduction and an increase in values, in reply to any challenge. They are categorized into positive and negative APPs. The negative APPs comprise transferring and albumin, the most copious constitutive plasma protein. The positive one is glycoproteins created mostly by hepatic cells upon stimulation by pro-inflammatory cytokines and released into blood stream. The positive APPs include, Haptoglobin, C-reactive protein, serum amyloid A, alpha- 1-acid glycoprotein, fibrinogen and ceruloplasmin .
Little is known about the use of lipoprotein profile and Alpha- 1-acid glycoprotein in cases of BRD in calves, which is the main objective of the current study.
Materials and Methods
Twenty-five Holstein calves (2-4 month old) from a private farm in Harad region, Saudi Arabia with clinical picture of BRD including fever, polypnea, nasal discharge, dyspnea, crackles on chest auscultation and loss of appetite were selected to the current investigation. In addition, ten clinically healthy calves were selected as a control group. Blood, nasal and bronchoalveolar lavage were obtained from all calves under investigation.
Two types of blood samples were collected from calves under investigation. The first blood samples were collected on heparinized tube and the second was collected on plain tubes for obtaining clear sera. Complete blood picture were determined using VetScan HM5 Hematology system.
Serum enzymes including AST, ALT, and ALP were estimated according to the methods previously described by Kachmar and Moss (1987) , Bergmeyer and Harder (1986)  and Varley et al. (1980) > respectively. The levels of total serum protein, albumin, TAG, LDL-c, HDL-c and total cholesterol were determined according to the methods previously described by Doumas et al., (1981) ; Henry (1966) ; Fossati and Prencipe (1982) ; Friedwald et al. (1972) ; Demacker et al. (1980)  and Richmound (1973) , respectively. In addition, VLDL-c was calculated by division of TAG/5 mg dL-1 .
Serum Alpha-1-acid glycoprotein (AGP) was estimated using a commercial radial immune diffusion kit supplied by Ecos Institute (Furukawa, Miyagi, Japan). The procedure recommended by the manufacturer was monitored and the test outcome was read after 48 h incubation in a humid chamber at room temperature. The values of AGP were reported in mg/L.
All data was presented as mean ± standard error of mean by using student-t-test. All tests were performed using computer package of the statistical analysis system .
The laboratory results revealed a detected elevation in the values of leukocytic count and neutrophil percentage in calves with pneumonia when compared with control groups (Table 1). Moreover, there was a significant elevation in the levels of TAG, LDL-c, ALT, AST, ALP and AGP with significant decrease in the levels of total protein, albumin, cholesterol, HDL-c in pneumonic calves when compared with control calves (Table 2 and Figure. 2). The bacteriological examination of nasal swabs and bronchoalveolar lavage in calves under investigation revealed a presence of predominant two classes of microorganisms shared in induction of pneumonia in calves. These microorganisms were Pasteurella spp., (72%), and Escherichia coli (28%) as shown in Figure 1.
Figure 2. Boxplot of AGP levels in control (0) and pneumonic calves (1) AGP
In this study, investigation of the utility of AGP and lipid profile in diagnosis of cases of BRD in calves were carried out.
The detected (P ≤ 0.05) higher levels of total leucocytic count and neutrophils percentage in pneumonic calves (Table 1) may be endorsed to a range of immunomodulatory effects . Former investigations [5, 10] stated such elevation in total leucocytic count in calves with bacterial pneumonia. Furthermore, the elevated leucocytic count was recorded in many infectious diseases [22,5]. From the other side, the detected elevation of AST, ALT, and ALP levels and decreased liver albumin synthesis in pneumonic calves (Table 2) may be allied with possible hepatic dysfunction persuaded by inflammatory response (pneumonia). Nikolic et al. (2006)  detected comparable higher values of ALT, AST and ALP in rats and Civelek et al., (2007)  in neonatal calves.
The consequence of inflammatory reaction on hepatic biosynthesis of albumin still debatable . Conversely, decreased albumin values observed in this investigation comes in concurrence with earlier findings stated by Civelek et al., (2007). Comparable significant decrease in the HDL-c and total cholesterol values, accompanied by significant higher levels (P ≤ 0.05) of VLDL-c and triglycerides of pneumonic calves were formerly detected in patients with septic infection [2, 16] and pneumonic buffalo-calves . The decreased values of serum cholesterol in pneumonic calves may be ascribed to subsequent changes in either lipoprotein metabolism or liver dysfunction or inflammatory processes . Decreased values of HDL-c possibly ascribed to its protective effects against inflammation which intermediated via bacterial endotoxins binding and subsequent neutralization . Moreover, it was stated that inflammation leads to hypertriglyceridemia in both animals and human [1, 26]. This may be attributed to an increased synthesis of VLDL-c, diminished conversion of VLDL-c to LDL-c by the embarrassment of lipoprotein lipase action  or stimulation of hepatic and adipose tissue lipolysis as well as hepatic fatty acid synthesis, which serve as substrates for hepatic VLDL synthesis .
The primary way leading to significant increase in APP sin infected calves in this study, may involve initial secretion of inflammatory cytokines by macrophages at the site of inflammation or infection. This results in a cascade of additional secretion of cytokines by macrophages and other immune cells. The most important stimulator of APPs are cytokines particularly, IL-1, IL-6 families and TNF .
Bacterial invasion stimulate a potent acute phase reaction inside the animal body whereas viral infections generally lead to weak or non-detectable acute phase reactions .
Inflammation leads to secretion of inflammatory cytokines like IL-1, IL-6 and tumor necrosis factor (TNF) which change the blood levels of a multiplicity of proteins that are created mostly in the hepatic cells . The levels of these proteins is usually varied from low to non-detectable in healthy animals and elevated levels are used to diagnose and monitor animal diseases [18, 11].The precise type of APPs and the time course for changes in these proteins differ by species based on the starting signal or causal inflammatory process .
The exact role of Alpha-1-acid glycoprotein (AGP) is not yet clear, however it binds to a variety of metabolites like histamine, heparin, and serotonin, catecholamine and steroids . Moreover, it was reported that, AGP binds to pharmacological compounds, which may have therapeutic consequences as the amount bound can influence the metabolically active fraction of the drug. Elevated serum levels of AGP because of acute phase reaction may be due to its effect in reducing the levels of free drugs, thus influencing their pharmacokinetics.
Elevated levels of AGP in pneumonic calves in this study may be due to the involvement of AGP in plasma transport protein and immunomodulation of the inflammatory reply. Moreover, AGP may further defend the calves against invading bacteria, and acts as chaperone .
The binding and delivery prosperity of AGP is notable; assumed the contextual, that AGP could distinctly elevate its values during acute phase response, thus becoming one of the most plentiful proteins in serum [29, 9].
AGP has been categorized in a subset of lipocalins, the so-called immunocalins, a subfamily of proteins that may additionally regulate the inflammatory response against invading pathogen .
From the present study, it could be concluded that lipoprotein profile and alpha-1-acid glycoprotein could be used as diagnostic markers for pneumonia in calves.
The authors would like to thank King Abd Al-Aziz City for Science and Technology (KACST) for their financial support (Project number AT-32-85) of the research and their unlimited assistance throughout the project period.
- Alvarez C, A. Ramos (1986) Lipids, lipoproteins, and apoproteins in serum during infection. Clin. Chem 32: 142-145.
- Amersfoort E.S.V, T.J.C.V Berkel, J. Kuiper (2003) Receptors, mediators and mechanisms involved in bacterial sepsis and septic shock. Clin. Microbiol. Rev. 7: 379-414.
- Bauer JD (1982) Clinical Laboratory Methods. (9th Edtn), Mosby. 1235.
- Bergmeyer HU, M Harder (1986) A colorimetric method of the determination of serum glutamic oxaloacetic and glutamic pyruvic transaminase. Clin. Biochem. 24: 481-481.
- Civelek T, K Kav, I Camkerten, AH Celik, A Acar (2007) Effect of bacterial pneumonia inneonatal calves on serum lipids. Bull. Vet. Inst. Pulawy 51: 503-507.
- Demacker PM, HE Von-Janssen, AM Hifman, AV Lear, AP Jansen (1980). Measurement of high-density lipoprotein cholesterol in serum: Comparison of six isolation methods combined with enzymic cholesterol analysis. Clin. Chem. 26: 1780-1786.
- Doumas BT, DD Bayse, RJ Carter, T Peters, R Schaffer (1981) A candidate reference method for determination of total protein in serum. I. Development and validation. Clin. Chem., 27: 1642- 1650.
- Eckersall PD, Bell R (2010) Acute phase proteins: Biomarkers of infection and inflammation in veterinary medicine. Vet J. 185(1):23-7.
- Eckersall PD, Young FJ, McComb C, Hogarth CJ, Safi S, et al. (2001) Acute phase proteins in serum and milk from dairy cows with clinical mastitis. Vet. Rec. 148:35-41.
- El-Bahr SM, EL-Deeb WM (2013) Acute Phase Proteins, Lipid Profile and Proinflammatory Cytokines in Healthy and Bronchopneumonic Water Buffalo Calves. American Journal of Biochemistry and Biotechnology 9 (1):34-40.
- El-Deeb WM, Iacob OC (2012) Serum acute phase proteins in control and Theileria annulatainfected water buffaloes (Bubalus bubalis).Veterinary Parasitology 190: 12– 18.
- El-Ghmati SM, EMV Hoeyveld, JGV Strijp, JL Ceuppens, EA Stevens (1996) Identification of haptoglobin as an alternative ligand for CD11b/ CD18. J. Immunol. 156: 2542-2552.
- Feingold KR, I. Staprans, RA Memon (1992) Endotoxin rapidly induces changes in lipid metabolism that produce hypertriglyceridemia: Low doses stimulate hepatic triglyceride production while high doses inhibit clearance. J. Lipid Res 33: 1765- 1776.
- Feldman BF, JG Zinkl, NC Jain (2000) Schalm’s Veterinary Hematology. (5th Edtn), Blackwell. 1344.
- Fossati P, I Prencipe (1982) Serum triglyceride determination calorimetrically with an enzyme that produce hydrogen peroxide. Clin. Chem. 28: 2077- 2083.
- Fraunberger P, S Schaefer, K Werdan (1999) Reduction of circulating cholesterol and apolipoprotein levels during sepsis. Clin. Chem. Lab. Med. 37: 357-362.
- Friedwald WT, RT Levy, DS Fredrickson (1972) Estimation of the concentration of low-density lipoprotein cholesterol in plasma without use of the preparative ultracentrifuge. Clin. Chem. 8: 499-505.
- Glass EJ, Craigmile SC, Springbett A, Preston PM, Kirvar E, et al. (2003) The protozoan parasite, Theileria annulata, induces a distinct acute phase protein response in cattle that is associated with pathology. Int. J. Parasitol 33:1409-1418.
- Gouni I, K Oka, J Etienne (1993) Endotoxin induced hypertriglyceridemia is mediated by suppression of lipoprotein lipase at a posttranscriptional level. J. Lipid Res 4: 139-146.
- Henry RJ (1966) Clinical Chemistry. (1st Edtn), Harper and Row Publishers, New York.
- Israili ZH, Dayton PG (2001) Human Alpha-1-Glycoprotein and its interactions with drugs. Drug Metabolites Revue 33(2): 161-235.
- LaMonica CR, M Blackston, RB Dawson (1981) Acute renal failure associated with the thrombocytopenia of septicemia. Adv. Shock Res. 6: 75-79.
- Lögdberg L, Wester L (2000) Immunocalins: a lipocalin subfamily that modulates immune and inflammatory responses. Biochemical and Biophysical Acta 1482(1-2): 284-297.
- Nikolic J, I Stojanovic, R Pavlovic (2006) The role of L-arginine in toxic liver failure: interrelation of arginase, polyamine catabolic enzymes and nitricoxide synthase. Amino Acids 32: 127-131.
- O’Leary MJ, M Koll, CN Ferguson (2003) Liver albumin synthesis in sepsis in the rat: influence of parenteral nutrition, glutamine and growth hormone. Clin. Sci. 105: 691-698.
- Phetteplace HW, N Sedkova, KI Hirano (2000) Escherichia coli sepsis increases hepatic apolipoprotein B secretion by inhibiting degradation. Lipids 35: 1079-1085.
- Richmound W (1973) Preparation and properties of cholesterol oxidase from Nacardia sp. and its application to enzymatic assay of total cholesterol in serum. Clin. Chem. 19: 1350-1356.
- (2002) Statistical Analysis System. (1st Edtn), SAS Institute Inc., Cary, NC, USA.
- Sheldon IM, Noakes DE, Rycroft A, Dobson H (2001) Acute phase protein response to postpartum uterine bacterial contamination in cattle. Vet Rec 148: 172–175.
- Urban – Chmiel R, Grooms DL (2012) Prevention and Control of Bovine Respiratory Disease. Livestock Sci 3:27-36.
- Varley H, A Gewenlock, M. Bell (1980) Practical Clinical Biochemistry. (5th Edtn), Williams Heinemann Medical Books, London. 741.
- Wu A, CJ Hinds, C Thiemermann (2004) High-density lipoproteins in sepsis and septic shock: Metabolism, actions and therapeutic applications.Shock. 21: 210-221.
- Kachmar JF, DW Moss (1987) Enzymes. In: Fundamentals of Clinical Chemistry, W.B. Saunders Co, Philadelphia PA. 666-672.