A Retrospective Evaluation Of Various Methods To Determine Vertical Loss In Full Mouth Rehabilitation Patients
M.Sai Teja Reddy1, Subhabrata Maiti2*, Keerthi Shashanka3
1 Saveetha Dental College, Saveetha Institute of Management and Technical Sciences, Saveetha University, Chennai -77.
2 Senior lecturer, Department of Prosthodontics, Saveetha Dental College, Saveetha Institute of Management and Technical Sciences, Saveetha University, Chennai -77
3 Senior Lecturer, Department of Prosthodontics, Saveetha Dental College, Saveetha Institute of Management and Technical sciences, Saveetha University, Chennai -77
*Corresponding Author
Subhabrata Maiti,
Senior lecturer, Department of Prosthodontics, Saveetha Dental College, Saveetha Institute of Management and Technical Sciences, Saveetha University, Chennai -77.
Tel: 9007862704
E-mail: subhabratamaiti.sdc@saveetha.com
Received: May 28, 2021; Accepted: June 16, 2021; Published: July 05, 2021
Citation:M.Sai Teja Reddy, Subhabrata Maiti, Keerthi Shashanka. A Retrospective Evaluation Of Various Methods To Determine Vertical Loss In Full Mouth Rehabilitation Patients. Int J Dentistry Oral Sci. 2021;8(6):3099-3104.doi: dx.doi.org/10.19070/2377-8075-21000631
Copyright:Subhabrata Maiti©2021. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution and reproduction in any medium, provided the original author and source are credited.
Abstract
The aim of this study was to retrospectively evaluate the commonly used technique for evaluating vertical dimension loss in patients who are required for full mouth rehabilitation among dental students. A total of 145 data entries were taken after reviewing 86000 patient data, duplicate and missing entries were omitted. So a total of 114 entries were evaluated. The data was collected from patient records in Saveetha Dental College, over a period of one year. The evaluation was based on the technique used by the dental students, to measure the loss of vertical dimension. The results of the study were subjected to statistical analysis. Data analysis was done using SPSS software version 23.0.Chi-square test and frequency evaluation was done to evaluate the most frequently used method to record vertical loss. It was found that about 93.3% of students have used the Niswonger and Thomson method and 6.7% have used the Tactile perception method although found to be statistically not significant. Pearson (Chi-Square Value - 8.559 and p-value - .638) (p>0.05)The present study concluded that most of the dental students preferred Niswonger and Thomson method for determining loss of vertical dimension.
2.Introduction
6.Conclusion
8.References
Keywords
Vertical Dimension; Techniques To Record Vertical Dimension; Tooth Supported Full Mouth Rehabilitation.
Introduction
Vertical dimension is the length of the face as determined by the
amount of separation of the jaws’[1]. Restoring Correct vertical
dimension of occlusion is one of the most important steps with
adequate function and esthetics’[2]. Restoring the vertical dimension
of face is one of the fundamental principles of the spherical
theory of occlusion to create a happier and more comfortable
patient, more efficient mastication, and a perceptible favorable
change in facial contour.Causes of vertical dimension loss is due
to abrasion, loss of all of the teeth, loss of molar support on
either or both sides, and the early loss of six year molars which
allows drifting of teeth.
Effects Of Decreased Vertical Dimensions
It will have a direct effect of a reduction of the vertical dimension
is the production of temporomandibular joint disturbances
including partial subluxation and those symptoms known as
Costen’s syndrome,symptoms of these include: impaired hearing,
noises in the ear, stuffiness of the ear, sinus disturbances, headaches,
burning of the side of the nose and throat, tenderness of
the temporomandibular joint on palpation, burning tongue and
vertigo, sometimes the meniscus perforates and gets damaged.
Although there are many controversies in literature .
Effects Of Increased Vertical Dimension
Pain and clicking in the temporomandibular joint, Increased lower
facial height, difficulty in swallowing and speech, stretching of
facial muscles, increased volume of the oral cavity and cheek bite
will also occur.
Importance in recording vertical jaw relation is given because any errors in this record produce the first sign of discomfort [3] .Vertical
jaw relation can be recorded in two positions, vertical dimension
at rest and vertical dimension at occlusion. VDO (vertical
dimension at occlusion) - it is the length of the face when the
teeth are in contact and the mandible is in centric relation and
the other is VDR (vertical dimension at rest), it is the length of
the face when the teeth are separated and the mandible is in a
physiologic rest position. Both vertical dimensions are subject to
change resulting from loss of teeth.
When at rest the tooth does not maintain contact at rest. The
space between the teeth at rest is called the free way space. The
free way space exists only at rest. During occlusion, the teeth
come in contact with one another and the space is lost. The vertical
dimension at occlusion (VDO) should always be 2 - 4 mm
lesser than the vertical dimension at rest (VDR). VD at rest = VD
at occlusion + freeway space [4].
Many patients have adapted to decreased vertical dimension due
to bone resorption and posterior tooth wear.One of the more
controversial aspects of jaw relation involves vertical rest position.
Establishment of a correct vertical dimension of occlusion
is of concern and it is considered essential to establish a correct
vertical rest position. Rest position acts as a reference point during
recording the vertical dimension at occlusion. Restoring the
proper vertical dimension is complicated because the rest position
may be subject to change[5].
According to Gottlieb, a German named Wallisch was one of
the first to define the physiologic rest position of the mandible.
In 1906, Wallisch described the mandibular rest position as that
position of the mandible wherein all muscle action is eliminated
and the mandible is passively suspended. He reported that in this
position the opposing teeth do not contact[6]. Boucher et al.’
noted that if the vertical dimension is too great the patient may
complain of soreness of the residual ridges, tightness of facial
muscles, and clicking of the dentures during speech. If the vertical
dimension is too small, the patient will look older as the lower
half of the face is compressed, the cheeks and lips are drooped
and chin protrudes.Altering the vertical dimension of occlusion
result in traumatic occlusion [7].
The physiologic rest position has been considered by some to
remain constant throughout life regardless of the presence or absence
of the teeth[8]. Swerdlow found that the vertical dimension
of rest varies after natural tooth contacts are lost. Also, the rest
vertical dimension can undergo a reduction comparable to the
loss of occlusal vertical dimension[9]. Atwood reported instability
of the rest position and a decrease in rest face height after
removal of occlusal contacts[5]A variety of techniques have been
proposed to determine measurements for the correct vertical dimension
of occlusion[8],[8,10], [11], [12]. Accuracy and repeatability
of the measurement, adaptability of the technique, type
and complexity of the equipment needed, and the length of time
required to secure the measurement are the criteria to be considered
when selecting the best method to use.
There are numerous beliefs and theories put forward as to the determination
of vertical dimension. Some believe that the vertical
dimension restored should be the same as probably what existed
prior to the edentulous situation[13]. Although many techniques
to determine the correct vertical dimension of occlusion have
been proposed like the use of pre extraction records, physiologic
rest position, closing forces (boos bimeter method), tactile sense,
phonetics, esthetic appearance, open rest method, facial measurements,
deglutition and the electromyographic method[14]. Finding
a reliable method to determine the correct vertical dimension
of occlusion has always been a challenge for the clinicians in the
field of complete denture prosthodontics.Previously our team has
a rich experience in working on various research projects across
multiple disciplines The [15-17][18-29].
Methods To Record Vertical Dimension At Rest [30] by using
Facial measurements after swallowing and relaxing, Tactile sense,
Measurements of anatomical landmarks, Speech and by analysing
facial expression.
Methods To Record Vertical Dimension At Occlusion include
Mechanical Methods Includes
?Ridge relation - by seeing the parallelism of ridges and measuring
distance from incisive papilla to mandibular incisors
?Pre extraction records includes Profile photographs, Profile
silhouettes, Radiography, Articulated casts and Facial measurements
?Measurements from another denture
Physiological Methods include
Using a Powerpoint, Using wax occlusal rims, Physiological rest
p[osition, Phonetics, Aesthetics, Swallowing method, Tactile sense
or neuromuscular perception and Patients perception of comfort.
The main objective of this study was to evaluate which type of
centric relation method is frequently used by the dental clinicians
in an institutional setting.
Materials And Methods
Sample Collection
Retrospective study has been conducted. A total of 145 patient
data were taken from 86000 of patient data after reviewing , duplicate
and missing entries were omitted. All the data was reviewed
from the Dias Data set between 01 June 2019 and 31 march 2020 ,
ethical approval was done by university ethical committee (SDC/
SIHEC/2020/DIASDATA/0619-03200).Samples with improper
data and repetitions of the data were excluded from the study.
Then the sample size has come to 114.The data is then arranged
and checked for the frequency of different methods used for recording
vertical loss in full mouth rehabilitation cases.
Inclusion Criteria
Patients with VD loss, who require full mouth rehabilitation, patients
with informed consent and patients with good neuromuscular
coordination.
Exclusion Criteria
Patients without informed consent and Complete edentulous patients.
Statistical Analysis
The results of the study were subjected to statistical analysis. Data
analysis was done using SPSS software. Frequency evaluation and
Chi-square test was done to evaluate the type of finish line configuration
given to various teeth.
Dependable Variables include the type of technique used to determine
vertical loss and the practitioner.
Independent Variables include the age, gender, type of material
and technique of fabrication used.
Results And Discussion
From the retrospective study, the frequency of students using different
types of techniques to determine the vertical loss was Tactile
perception method has a count of 9 and the Niswonger and
Thomson method with a count of 105.(Table 1)
The bar chart shows the percentage of various techniques used to
determine vertical loss. Niswonger and Thomson technique was
used by 92.11% students and Tactile perception technique was
used by 7.89% students.(Figure 1)
Out of all the data collected, 3rd year postgraduates reported the
highest percentage of cases followed by 2nd year postgraduates
and 1st year postgraduates with a percentage of 64.91, 29.82 and
5.26.(Figure 2)
In 1st year postgraduate students all of them have used Niswonger
and Thomson method to record vertical dimension, in
2nd year postgraduates, 3 have been reported using tactile perception
method and 31 have been use Niswonger and Thomson
method and among 3rd year postgraduates, 6 have been reported
using tactile perception method and 68 have been use Niswonger
and Thomson method.(Table 2)
The bar diagram shows the percentage of various techniques used
by various postgraduates to record VD. In 1st year postgraduates
students all of them have used Niswonger and Thomson method
to record vertical dimension with an overall percentage of 5.26, in
2nd year postgraduates, 2.63% of them have been reported using
tactile perception method and 27.19% have been use Niswonger
and Thomson method and among 3rd year postgraduates, 5.26%
have been reported using tactile perception method and 59.65%
have been use Niswonger and Thomson method.(Figure 3)
The determination and establishment of vertical dimension has
always been a challenge to the prosthodontist in different eras, as
it is the most significant and intricate step in the construction of a
complete denture for the rehabilitation of an edentulous patient.
This has ultimately led to establishing the vertical dimension by
employing various means. Methods to establish the occlusal vertical
dimension can either be subjective or objective. The subjective
methods comprise evaluation of esthetics, phonetics, swallowing
and patient comfort. The objective methods comprise electromyographic
records, biting power and the utilization of facial
measurements.
Still no accurate method of assessing the vertical dimension of
occlusion in edentulous patients is available to the dentists[31].
Several procedures have been developed in an attempt to establish
a clinically useful, reproducible rest position. Gillis’ asked the
patient to pronounce the letter “M” while sitting upright. He believed
that the mandible assumes the lowest position of the speaking
space in this manner. Others recommend recording a vertical
rest position after the patient is asked to wet the lips, say “Mississippi”
and then hold that position.” These “phonetic” procedures
have provided clinicians with fairly reproducible measures which
have been found useful as a guide in denture construction and
other dental procedures[32].
Hickey et al.’ reported that clinical rest position could be identified
through electromyographic (EMG) measurements. They
found that muscle activity was minimal at the clinical rest position
when measured by having subjects say “14” and swallow [33].
Yemm reviewed the literature on clinical rest position and concluded
that muscle activity was minimal at clinical rest position.
He postulated that the principal factor determining clinical rest
position was not tonic. Muscle activity but rather the result of an
equilibrium between the force of gravity and the elasticity of the
soft tissue. Little or no muscle activity was believed necessary to
maintain clinical rest position [34].
There are various procedures used for restoring vertical dimension,
some of which have proved to be very successful. In the
mouths of patients who have suffered complete destruction of all
identification of their original dental anatomy or jaw relationships,
the establishment of the teeth on the curved surface described by
Monson is most successful. The mandibular teeth are restored so
that their occlusal surfaces conform to a spherical surface with
a four inch radius. The center of this segment of the spherical
surface is in the region of the glabella, and the curvature of the
surface is concentric with the condyle paths. The upper teeth are
restored in such a manner that they harmonize and properly occlude
with the lower teeth. The restorations placed on the teeth
of the upper jaw govern the amount of vertical opening that is
established. Such an arrangement allows complete freedom of the
mandible, and also permits a more even distribution of the forces
of mastication.Our institution is passionate about high quality evidence based research and has excelled in various fields [35-45].
Figure 1 :- The bar graph shows the total percentage of different techniques used to record vertical dimension loss. X axis represents the technique used and Y axis represents the total percentage of different techniques used. Most commonly used method was the Niswonger and Thompson method (92.11%) when compared to the Tactile perception method.(7.89%)
FIGURE 2: The bar graph shows the frequency distribution of postgraduate students done with Full mouth rehabilitation cases. X axis represents the different years of postgraduate students in the department of prosthodontics and Y axis represents the total percentage of postgraduate students done with Full mouth rehabilitation cases. Purple colour represents 3rd year postgraduates, bluish green represents 2nd year postgraduates and dark grey colour represents 1st year postgraduate students.
Figure 3: The bar graph shows the association between type of technique for determining VD loss and their use among postgraduate students. X axis represents the different years of post graduate students and Y axis represents the total number of students using different types of techniques. Association between the type of technique and various postgraduate students was found to be statistically not significant.(Chi-Square Value - 8.559 and p-value - .638) (p>0.05). However, niswonger and thomson technique was most commonly used by 3rd year post graduate students.
TABLE 1: Table shows the frequency of techniques used to record vertical dimension (VD) loss. The most commonly used method was the Niswonger and Thompson method (92.11%) when compared to the Tactile perception method.(7.89%)
TABLE 2: The table shows the association of different techniques to record vertical dimension and different years of postgraduate students in full mouth rehabilitation cases. Association between the type of technique used and various postgraduate students was found to be statistically not significant.(Chi-Square Value - 8.559 and p-value - .638) (p>0.05).
Conclusion
Within the limitations of the study it can be concluded that Niswonger
and Thomson method was the most commonly used
technique to record the vertical dimension among all post graduate
students. Further extensive research can be conducted with
larger sample size and more reliable and less technique sensitive
methods should be used in recording vertical dimension.
Acknowledgement
This research was done under the research department of
Saveetha dental College and hospitals. We sincerely provide gratitude
and are very thankful to the guide who helped in making this
study possible.
Author Contributions
First author, Dr. Sai Teja Reddy performed the analysis, and interception
and wrote the manuscript. Second author, Dr.Subhabrata
Maiti contributed to conception , data design, analysis interpretation
and critically revised manuscripts. The third author, Dr.
Keerthi Sasanka Participated in the study revised the manuscript
as per guideline, alignments and formatting . All the authors have
discussed the results and contributed to the final manuscript.
References
- Sollenius O, Petrén S, Bondemark L. An RCT on clinical effectiveness and cost analysis of correction of unilateral posterior crossbite with functional shift in specialist and general dentistry. Eur J Orthod. 2019. Available from:
- Asiry MA, AlShahrani I. Prevalence of malocclusion among school children of Southern Saudi Arabia. J. Orthod. Sci. 2019;8:2.
- Yu X, Zhang H, Sun L, Pan J, Liu Y, Chen L. Prevalence of malocclusion and occlusal traits in the early mixed dentition in Shanghai, China. PeerJ. 2019 Apr 2;7:e6630.Pubmed PMID: 30972246.
- Bs P, Phulari BS, Rashanal A. Interceptive Orthodontics. Orthodontics: Principles and Practice. 2017.195. Available from:
- Millett D, Day P. Clinical Problem Solving in Orthodontics and Paediatric Dentistry E-Book. Elsevier Health Sciences; 2010 Oct 18:224.
- Kutin G, Hawes RR. Posterior cross-bites in the deciduous and mixed dentitions. Am. J. Orthod. 1969 Nov 1;56(5):491-504.
- Egermark-Eriksson I, Carlsson GE, Magnusson T, Thilander B. A longitudinal study on malocclusion in relation to signs and symptoms of craniomandibular disorders in children and adolescents. Eur J Orthod. 1990 Nov;12(4):399-407.Pubmed PMID: 2086260.
- Andrade AS, Gameiro GH, DeRossi M, Gavião MB. Posterior crossbite and functional changes: a systematic review. Angle Orthod. 2009 Mar;79(2):380- 6.
- Thilander B, Wahlund S, Lennartsson B. The effect of early interceptive treatment in children with posterior cross-bite. Eur J Orthod. 1984 Jan 1;6(1):25-34.
- Linder-Aronson S. Effects of adenoidectomy on mode of breathing, size of adenoids and nasal airflow. ORL J Otorhinolaryngol Relat Spec. 1973;35(5):283-302.Pubmed PMID: 4795962.
- . Hannuksela A, Väänänen A. Predisposing factors for malocclusion in 7-yearold children with special reference to atopic diseases. Am J Orthod Dentofacial Orthop. 1987 Oct;92(4):299-303.Pubmed PMID: 3477948.
- Hafeez N. Accessory foramen in the middle cranial fossa. Res J Pharm Technol. 2016;9(11):1880-2.
- Krishnan RP, Ramani P, Sherlin HJ, Sukumaran G, Ramasubramanian A, Jayaraj G, et al. Surgical Specimen Handover from Operation Theater to Laboratory: A Survey. Ann Maxillofac Surg. 2018 Jul-Dec;8(2):234-238. Pubmed PMID: 30693238.
- Somasundaram S, Ravi K, Rajapandian K, Gurunathan D. Fluoride Content of Bottled Drinking Water in Chennai, Tamilnadu. J Clin Diagn Res. 2015 Oct;9(10):ZC32-4.Pubmed PMID: 26557612.
- Felicita AS. Orthodontic extrusion of Ellis Class VIII fracture of maxillary lateral incisor - The sling shot method. Saudi Dent J. 2018 Jul;30(3):265- 269.Pubmed PMID: 29942113.
- Kumar S, Rahman R. Knowledge, awareness, and practices regarding biomedical waste management among undergraduate dental students. Asian J Pharm Clin Res. 2017;10(8):341.
- Gurunathan D, Shanmugaavel AK. Dental neglect among children in Chennai. J Indian Soc Pedod Prev Dent. 2016 Oct 1;34(4):364.
- Sneha S. Knowledge and awareness regarding antibiotic prophylaxis for infective endocarditis among undergraduate dental students. Asian J Pharm Clin Res. 2016 Oct 1:154-9.
- Dhinesh B, Lalvani JI, Parthasarathy M, Annamalai K. An assessment on performance, emission and combustion characteristics of single cylinder diesel engine powered by Cymbopogon flexuosus biofuel. Energy Convers. Manag. 2016 Jun 1;117:466-74.
- Choudhari S, Thenmozhi MS. Occurrence and Importance of Posterior Condylar Foramen. Res J Pharm Technol. 2016;9(8):11–43.
- Paramasivam A, Vijayashree Priyadharsini J, Raghunandhakumar S. N6- adenosine methylation (m6A): a promising new molecular target in hypertension and cardiovascular diseases. Hypertens Res. 2020 Feb;43(2):153- 154.Pubmed PMID: 31578458.
- Wu F, Zhu J, Li G, Wang J, Veeraraghavan VP, Krishna Mohan S, et al. Biologically synthesized green gold nanoparticles from Siberian ginseng induce growth-inhibitory effect on melanoma cells (B16). Artif Cells Nanomed Biotechnol. 2019 Dec;47(1):3297-3305.Pubmed PMID: 31379212.
- Palati S, Ramani P, Shrelin HJ, Sukumaran G, Ramasubramanian A, Don KR, et al. Knowledge, Attitude and practice survey on the perspective of oral lesions and dental health in geriatric patients residing in old age homes. Indian J Dent Res. 2020 Jan-Feb;31(1):22-25.Pubmed PMID: 32246676.
- Saravanan M, Arokiyaraj S, Lakshmi T, Pugazhendhi A. Synthesis of silver nanoparticles from Phenerochaete chrysosporium (MTCC-787) and their antibacterial activity against human pathogenic bacteria. Microb Pathog. 2018 Apr;117:68-72.Pubmed PMID: 29427709.
- Govindaraju L, Gurunathan D. Effectiveness of Chewable Tooth Brush in Children-A Prospective Clinical Study. J Clin Diagn Res. 2017 Mar;11(3):ZC31-ZC34.Pubmed PMID: 28511505.
- Vijayakumar Jain S, Muthusekhar MR, Baig MF, Senthilnathan P, Loganathan S, Abdul Wahab PU, et al. Evaluation of Three-Dimensional Changes in Pharyngeal Airway Following Isolated Lefort One Osteotomy for the Correction of Vertical Maxillary Excess: A Prospective Study. J Maxillofac Oral Surg. 2019 Mar;18(1):139-146.Pubmed PMID: 30728705.
- Almeida AB, Leite IC. Orthodontic treatment need for Brazilian schoolchildren: a study using the Dental Aesthetic Index. Dental Press J Orthod. 2013;18:103-9.
- Chowdhury MS, Sultana N, Naim MA, Nashrin T, Nahar L. Prevalence of Cross Bite among the Orthodontic Patients at a Dental Unit of Bangladesh. J Natl Inst Neurosci Bangladesh. 2019 Sep 7;5(2):167-71.
- Popovic N, Drinkuth N, Toll DE. Prevalence of class III malocclusion and crossbite among children and adolescents with craniomandibular dysfunction. J. orofac. Orthop./Fortschr. Kieferorthop. 2014 Jan 1;75(1):36-41.
- Bilgiç F, Gelgör IE. Prevalence of temporomandibular dysfunction and its association with malocclusion in children: an epidemiologic study. J. Clin. Pediatr. Dent. 2017;41(2):161-5.
- Vijayashree Priyadharsini J. In silico validation of the non-antibiotic drugs acetaminophen and ibuprofen as antibacterial agents against red complex pathogens. J Periodontol. 2019 Dec;90(12):1441-1448.Pubmed PMID: 31257588.
- Pc J, Marimuthu T, Devadoss P, Kumar SM. Prevalence and measurement of anterior loop of the mandibular canal using CBCT: A cross sectional study. Clin Implant Dent Relat Res. 2018 Apr 6;20(4):531-4.
- Ramesh A, Varghese S, Jayakumar ND, Malaiappan S. Comparative estimation of sulfiredoxin levels between chronic periodontitis and healthy patients - A case-control study. J Periodontol. 2018 Oct;89(10):1241-1248.Pubmed PMID: 30044495.
- Ramadurai N, Gurunathan D, Samuel AV, Subramanian E, Rodrigues SJ. Effectiveness of 2% Articaine as an anesthetic agent in children: randomized controlled trial. Clin Oral Investig. 2019 Sep;23(9):3543-50.
- Sridharan G, Ramani P, Patankar S, Vijayaraghavan R. Evaluation of salivary metabolomics in oral leukoplakia and oral squamous cell carcinoma. J Oral Pathol Med. 2019 Apr;48(4):299-306.
- Ezhilarasan D, Apoorva VS, Ashok Vardhan N. Syzygium cumini extract induced reactive oxygen species-mediated apoptosis in human oral squamous carcinoma cells. J Oral Pathol Med. 2019 Feb;48(2):115-121.Pubmed PMID: 30451321.
- Mathew MG, Samuel SR, Soni AJ, Roopa KB. Evaluation of adhesion of Streptococcus mutans, plaque accumulation on zirconia and stainless steel crowns, and surrounding gingival inflammation in primary molars: randomized controlled trial. Clin Oral Investig. 2020 Sep;24(9):1-6.Pubmed PMID: 31955271.
- Samuel SR. Can 5-year-olds sensibly self-report the impact of developmental enamel defects on their quality of life? Int J Paediatr Dent. 2021 Mar;31(2):285-286.Pubmed PMID: 32416620.
- R H, Ramani P, Ramanathan A, R JM, S G, Ramasubramanian A, et al. CYP2 C9 polymorphism among patients with oral squamous cell carcinoma and its role in altering the metabolism of benzo[a]pyrene. Oral Surg Oral Med Oral Pathol Oral Radiol. 2020 Sep;130(3):306-312.Pubmed PMID: 32773350.
- Chandrasekar R, Chandrasekhar S, Sundari KKS, Ravi P. Development and validation of a formula for objective assessment of cervical vertebral bone age. Prog Orthod. 2020 Oct 12;21(1):38.Pubmed PMID: 33043408.
- Vijayashree Priyadharsini J, Smiline Girija AS, Paramasivam A. In silico analysis of virulence genes in an emerging dental pathogen A. baumannii and related species. Arch Oral Biol. 2018 Oct;94:93-98.Pubmed PMID: 30015217.